FIELD: analytical methods in environmental monitoring.
SUBSTANCE: invention relates to water toxicology and sanitary hydrobiology and can be used in estimation of toxicity of water when performing testing of effluent and natural fresh waters. Method envisages placing groups of mollusks (Viviparus viviparous L) in test (experimental) and pure (control) water to stand for a certain amount of time, after which lever of these mollusks is prepared and homogenized to obtain further extract of lever proteins, wherein activity of hydrolytic enzymes, acid phosphatase and DNAse, is determined. Toxicity of water is correlated with reliable change in total activity of test enzyme or at least one qualitative change in multiple forms in test variant relative to control.
EFFECT: achieved rapidity and versatility of method with respect to various toxicants and enabled detection of contamination of water with dangerous substances in concentrations from one tenth to hundred limiting permissible levels and higher.
5 dwg, 9 tbl, 7 ex
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Authors
Dates
2007-10-20—Published
2006-06-02—Filed