FIELD: molecular biology, biotechnology, medicine, pharmaceutical industry.
SUBSTANCE: invention proposes RNA molecule able to target-specific interference of RNA and represents a double-stranded RNA molecule of size 19-22 nucleotides and having 3'-bulge consisting of 1-5 nucleotides This RNA is prepared by combining two chains of RNA and used for preparing a pharmaceutical composition. After incorporation into the multicellular eucaryotic organism this RNA molecule provides promotion to target-specific interference of RNA and results to decreasing level of expression of the gene-target or to knockout of the gene-target. Method for promotion of target-specific interference of RNA using this RNA molecule is used in assay and modulation of gene function. Cell containing endogenous nucleic acid-target, RNA molecule able for target-specific RNA interference and exogenous nucleic acid-target is used in analytical procedures. Using the invention provides silence of the gene-target mediated by target-specific interference of RNA.
EFFECT: valuable biological properties of RNA.
40 cl, 24 dwg, 3 ex
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