METHOD OF PRODUCTION OF IMMONUACTIVE POLYPEPTIDES/PEPTIDES HAVING IMMUNOMODULATING PROPERTIES Russian patent published in 2008 - IPC A61K35/12 A61P37/02 

FIELD: medicine; immunology.

SUBSTANCE: method implies that animals' thymus, e.g. sea animals' thymus, is defatted and connective tissue cleaned, milled, lyophilised, degreased with liquid CO₂, hydrated in isotonic phosphate buffer at pH 7.2-7.4, sediment is removed by centrifugation, supernatant is collected and warmed up at 80°C during 10-15 min, centrifugated, supernatant is collected and pH is reduced to 4.0-4.2, deposited with ammonium sulphate, formed sediment is removed by centrifugation, dissolved in tris-HCl buffer, pH 8.0-8.2, ammonium sulphate is removed on column with Sephadex G-15, material of column outer content is, lyophilised, dissolved in 0.01 M tris-HCl, buffer containing 0.14 M NaCl, pH 8.0-8.2, gel- chromatographed on column with Sephadex G-50, end product is collected by molecular weight 500 to 25000 dalton, lyophilised, dissolved in bidistilled water and desalted on column with Sephadex G-15, end product produced in column free content is lyophilised.

EFFECT: method provides higher product yield and storage stability.

1 tbl, 4 ex