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2 354 696

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(51)

IPC

C12N9/14(2006-01-01)

C12N9/48(2006-01-01)

C12N9/64(2006-01-01)

(21) (22)

Application

2007129960/13, 2007-08-07

(24)

Start date

2007-08-07

(22)

Actual filing date

2007-08-07

(45)

Published

2009-05-10

(72)

Inventor

Biketov Sergej FedorovichReshetnjak Tat'Jana ViktorovnaRepolovskaja Tat'Jana VladimirovnaEvsegneev Sergej Ivanovich

(73)

Holder

Federal'Noe Gosudarstvennoe Uchrezhdenie Nauki Gosudarstvennyj Nauchnyj Tsentr Prikladnoj Mikrobiologii I Biotekhnologii Gnts Pmb)

METHOD OF OBTAINING CARBOXYPEPTIDASE B FROM PIG PANCREAS Russian patent published in 2009 - IPC C12N9/14 C12N9/48 C12N9/64

Abstract RU 2354696 C1

FIELD: medicine.

SUBSTANCE: for separating carboxypeptidase B from pig pancreas extraction, precipitation by ammonium sulphate and purification are carried out in buffer solutions, which contain 0.001-0.005 M of zinc chloride. Purification is carried out by double chromatography on Q-sepharose, and during chromatography process elution of 0.07-0.08 M NaCl in 0.005 M tris HCI buffer, pH 7.6, is used.

EFFECT: invention ensures simplification of method of carboxypeptidase B obtaining preserving high specific activity of final medication and carboxypeptidase B stability.

5 ex

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RU 2 354 696 C1

Authors

Biketov Sergej Fedorovich

Reshetnjak Tat'Jana Viktorovna

Repolovskaja Tat'Jana Vladimirovna

Evsegneev Sergej Ivanovich

Dates

2009-05-10—Published

2007-08-07—Filed