METHOD OF PRODUCING CARBOXYPEPTIDASE IN THE PIG'S PANCREAS Russian patent published in 2018 - IPC C12N9/14 C12N9/48 C12N9/64 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology. Method for producing carboxypeptidase B from pig pancreas involves homogenization of animal feed, autolysis, double-volume extraction of a buffer solution containing 0.01 M KH₂PO₄ 0.001 M ZnCl₂ at a temperature of 65 °C to obtain an extract. Ammonium sulfate is precipitated in a predetermined ratio and centrifuged. Precipitate is dissolved in a 0.05 M Tris-HCl buffer containing 0.001 M ZnCl₂, filtered through a glass fiber and cardboard filters at the specified temperature parameters. Filtrate is diluted in 0.005 M Tris-HCl buffer containing 0.001 M ZnCl₂, followed by ultrafiltration in a tangential flow on a membrane cassette module of polyethersulfone with a cut-off limit of 20 kDa and double ion-exchange chromatography on a column with UNO Shhere. Balanced with 0.005 M Tris-HCl buffer containing 0.01 NaCl and on a Macro Prep Q column equilibrated with the same buffer.

EFFECT: invention allows to increase the yield of the enzyme and its activity.

1 cl, 2 ex