FIELD: pharmacology.
SUBSTANCE: nutrient medium for mammal cell cultivation contains balanced salt solution, a source of nutrients and growth-stimulating factors as a vegetable hydrolysate, amino acids and vitamins. As the vegetable hydrolysate, it contains an enzymatic hydrolysate of rice flour with with pH 6.2-6.7, content of amine nitrogen 2.2-2.8 wt %, residual nitrogen 3.0-4.2 wt % and/or soya flour with pH 6.2-6.7, content of amine nitrogen 2.9-3.5 wt %, residual nitrogen 5.6-6.6 wt % at the following content of components: enzymatic hydrolysate of rice and/or soya flour 3-20 g/l, amino acids 0.11-0.86 g/l, vitamins 0.55-22.5 mg/l, balanced salt solution to 1 l.
EFFECT: standardising and reduction in price of nutrient medium, specially for cultivation of MDCK and Vero cells used in manufacturing of culture influenza virus vaccines.
4 cl, 3 tbl, 4 ex
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Authors
Dates
2009-12-27—Published
2008-06-11—Filed