FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology and can be used as an anti-mosquito agent. The method involves growing a Calcarisporium arbuscula F-80 fungus strain which is deposited in the collection of microorganisms of the State Research Centre of applied microbiology and biotechnology on Chapek+ solid medium at 23°C for 12 days until formation of a continuous aerial mycelium with adult colonies with subsequent culturing at the same temperature on a medium containing soya flour, yeast extract, glucose, KH2PO4, Mg2SO4 for 4 days until formation of a blastospore. The obtained culture liquid is centrifuged to obtain a centrifuge effluent. The obtained centrifuge effluent is extracted with methylene chloride on a continuous liquid-liquid extract for 36 hours. The obtained extract is purified with solvents of different polarity (hexane, chloroform, ethylacetate, methanol) with subsequent flash chromatography and subsequent high pressure liquid chromatrography to obtain Aurovertin B which has mosquitocide properties.
EFFECT: invention widens the range of agents for making mosquitocide preparations.
3 ex, 4 tbl
Title | Year | Author | Number |
---|---|---|---|
AGENT HAVING MOSQUITOCIDE PROPERTIES | 2008 |
|
RU2391390C2 |
ENTOMOPHTHORA LAIRIDII N.SP. STRAIN ENTOPATHOGENIC FINGUS | 0 |
|
SU589700A1 |
STRAIN BACILLUS THURINGIENSIS VAR. ISRAELENSIS № 7-1/23A USED AS AGENT FOR PRODUCING PREPARATION HAVING LARVICIDAL ACTIVITY ON BLOOD-SUCKING MOSQUITOES | 2013 |
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STRAIN OF BACTERIUM BACILLUS SPHAERICUS DESIGNATED FOR PREPARING THE PREPARATION AGAINST MOSQUITOS | 1994 |
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METHOD TO CONTROL LARVAE OF BLOODSUCKING INSECTS | 2010 |
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RU2571938C1 |
STRAIN OF BACTERIUM BACILLUS THURINGIENSIS VARIETAS ISRAELENSIS DESIGNATED FOR PREPARING A PREPARATION EXHIBITING ACTIVITY AGAINST MOSQUITO LARVAE | 1997 |
|
RU2122791C1 |
CRYSTAL-FORMING BACTERIAL STRAIN BREVIBACILLUS LATEROSPORUS WITH A WIDE SPECTRUM OF ANTAGONIST ACTIVITY AND USE THEREOF | 2018 |
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POLYFUNCTIONAL BIOPREPARATION WITH WIDE SPECTRUM OF ANTAGONIST ACTIVITY AND APPLICATION THEREOF | 2019 |
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RU2733140C1 |
STRAIN OF ENTOMOPATHOGENIC FUNGUS BEAUVERIA BASSIANA FOR PROTECTION OF AGRICULTURAL PLANTS FROM INSECTS AND MITES, PLANT PESTS | 2020 |
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RU2751916C1 |
Authors
Dates
2010-06-10—Published
2008-02-21—Filed