METHOD OF DETECTING 2-METHOXY-4-ALLYLHYROXYBENZENE IN BIOLOGICAL MATERIAL Russian patent published in 2010 - IPC G01N33/15 G01N33/50 G01N30/00 

FIELD: chemistry.

SUBSTANCE: in the method, a biological object is infused with ethylacetate twice for 30 minutes each time. Ethylacetate extractions are separated, combined and dehydrated. The combined extraction is first evaporated in an air current to a small volume and then in a nitrogen current until complete evaporation of the solvent. The residue is dissolved in a system of solvents, undergoes chromatography in a macro-column with sorbent using a multicomponent mobile phase. Eluate fractions containing 2-methoxy-4-allylhydroxybenzene are combined and evaporated. Before chromatography, the residue is dissolved in hexane, extracted, separated, acidified, saturated with sodium sulphate and extracted with diethyl ether. The ether extraction is separated, dehydrated, evaporated until complete removal of solvent. The residue is dissolved in a mixture of solvents - hexane - dioxane - proanol-2 (40:5:1). Determination is carried out through chromatography-mass spectrometry using a capillary macro-column. The amount of 2-methoxy-4-allylhydroxybenzene is determined from the area of the chromatographic peak.

EFFECT: increased sensitivity of determination.

3 tbl, 2 ex