FIELD: analytical chemistry.
SUBSTANCE: analysis of a sample involves its milling, infusion with acetone, filtration of acetone extraction, concentrating and purification by chromatography method. The concentration is purified in column by using a normal-phase sorbent silica gel L 40/100μ and a mixture hexane-acetone (8:2) as a mobile phase. The following chromatography determination is carried out by HPLC method in column filled with sorbent "Nova-Pak C-18" using a mixture acetonitrile-water (7:3) as a mobile phase and a detector based on photodiode array. Method provides enhancing sensitivity of assay. Invention can be used in practice of sanitary-epidemiological stations, chemical-toxicological, veterinary and ecological laboratories.
EFFECT: improved assay method.
3 tbl
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| METHOD OF DETECTING 2-METHOXY-4-ALLYLHYROXYBENZENE IN BIOLOGICAL MATERIAL | 2008 |
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| METHOD OF DETERMINING 3-METHOXYHYDROXYBENZENE IN BIOLOGICAL MATERIAL | 2013 |
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| METHOD OF DETERMINING HYDROXYBENZENE AND ITS MONOMETHYL-SUBSTITUTED IN BIOLOGICAL MATERIAL | 2014 |
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| METHOD FOR BIOLOGICAL MATERIAL ANALYSIS FOR TETRAMETHYLTHIURAMSULPHIDE | 2009 |
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RU2456597C1 |
