METHOD OF OBTAINING BIOLOGICALLY ACTIVE TISSUE PREPARATION Russian patent published in 2010 - IPC A61K35/50 A61P15/00 A61P17/02 

FIELD: medicine.

SUBSTANCE: invention relates to veterinary. Method of obtaining biologically active tissue preparation based on cows' placenta includes filtration, crushing, homogenisation, centrifugation, pregnant uterus being removed in the first half of pregnancy (4-5 months) in period of evisceration of incalvers at slaughterhouse, longitudinal cut of serous-muscular-mucous layer of pregnant uterine horn is performed, cotyledons are collected by removing them from caruncles, preserving integrity of villi, cutting cotyledons with curved sterile scissors from tissue of baby placenta, puncturing with a needle chorionically amniotic membrane by vacuuming amniotic fluid is collected from amniotic bubble, cut cotyledons are crushed by cutting, and after that in homogeniser, earlier collected from the same uterus amniotic fluid is added in amount 10:1 of homogeneous mass, mixed slowly and settled, after that centrifuged at 10 thousand rev/min for 10 minutes, conservation is performed, supernatant part is poured into vials and sealed.

EFFECT: invention ensures increase of preparation efficiency.

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