METHOD FOR PRODUCING LIVE CELL VACCINE FOR BREAST CANCER PREVENTION Russian patent published in 2010 - IPC C12N5/00 A61P35/00 

FIELD: medicine.

SUBSTANCE: murine benign spontaneous breast tumour cells are cultivated. At first, the cultivation procedure is in vitro in the DMEM/RPMI-1640 medium with 10 % of bovine embryo serum up to 20 passages. Then, in vivo up to 22 passages. Then, once again in vitro up to 25 passages. Then, 10 thousand-1 million produced cells are introduced in a connective tissue capsule formed in the Balb/c mice after the introduction of a biocompatible gel. The tumour growth is enabled. The encapsulated tumour is aseptically removed and trypsinised to produce primary epithelial cell cultures. The produced cells concentrated 10 thousand-1 million are introduced into the capsule subcutaneously gel-formed in the Balb/c mice. The tumour growth is enabled for vaccination. In the in vitro culture, the cells are transplanted once a week while being disseminated in the ratio 1:3 - 1:4, with a monolayer twice washed with EDTA solution and removed with 0.25% trypsin during 2-3 min at room temperature, and the trypsinisation process is terminated by adding 10 % of fetal serum "к.р.с.".

EFFECT: invention allows producing an anticancer vaccine application of which is based on a principle of transplantation of a live pathological material to a person from an animal with similar disease.

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