FIELD: chemistry.
SUBSTANCE: method of analysing feed yeast to determine content of the nucleotide fraction involves depositing protein with copper sulphate in an alkaline medium, extraction thereof into a copper-protein complex, and subsequent determination of the amount of protein in two identical weighed amounts of one analysed sample, wherein in the first weighed amount, the weight ratio of proteins is determined from optical density of the protein selectively dyed with Folin's reagent. Protein is extracted from the second weighed amount by distilling ammonia from the copper-protein complex, determining total weight of nitrogen and using that value to calculate the weight ratio of protein, and the weight ratio of the nucleotide fraction is calculated as the difference between content of protein in the and second weighed amounts.
EFFECT: method enables preliminary rapid estimation of the weight ratio of nucleotides in an industrial product of microbiological synthesis of feed protein.
2 tbl, 1 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD OF QUANTITATIVE DETERMINATION OF TRUE PROTEIN IN NUTRIIENT YEAST | 0 |
|
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Authors
Dates
2011-03-10—Published
2009-08-21—Filed