FIELD: medicine.
SUBSTANCE: Bradyrhizobium japonicum BKM B-245 5D nodule bacterial strain used for manufacturing of a bacterial fertiliser stably improving soya seed crop by 3.0 centner/ha on the average. The strain is recovered from soya nodules of the mid-ripening variety October 70, grown up on brown forest soil of Svobodnensky District of the Amur Region. The seed grain is cultivated with using an agarised nutrient medium containing mannitol. Then it is placed in thermostats at temperature 27-28°C for 7 days. The seeds are inoculated on the soya seeding day with using a bacteria-molybdenum compound. The prepared 1.25 % molybdenum solution (ammonium molybdate 25 g/l) 2.5 ml is introduced in test tubes with streaks of the nodule bacterial strain being tested. A streak from a nutrient medium swarm is washed off with the molybdenum solution. By shaking a test tube, a homogeneous bakteria-molybdenum compound (2.5 ml) used for immediate processing of the soya seeds (250 g) is produced. Then, the soya seeds are intimated, dried in the shade and sowed in the prepared soil.
EFFECT: higher crop capacity and quality of the soya seeds, high adaptive capability.
1 tbl, 1 ex
Authors
Dates
2011-04-10—Published
2009-10-26—Filed