FIELD: medicine.
SUBSTANCE: claimed is method of removing protein molecules of external S-layer from surface of purple membranes of halobacteria Halobacterium salinarum cells, under impact of ultrasound. Method consists in the following: suspension of halobacteria cells is subjected to ultrasound impact, with frequency 880 kHz and energy density in medium 0.1 Wt/cm3, for 10 min at temperature 20°C.
EFFECT: method makes it possible to purify surface of purple membranes from bound with it protein complexes without breaking membrane integrity and without application of any other extraneous substances.
2 dwg, 4 ex
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METHOD OF IMMOBILISATION OF ORIENTED NATURAL PURPLE MEMBRANES OF HALOBACTERIA ON INTERNAL SURFACE OF TRANSPARENT POROUS TUBE-SHAPED PADDING AND FORMATION OF MULTI-LAYERED PACKETS OF PHOTOSENSIBLE BIOSTRUCTURES | 2008 |
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Authors
Dates
2011-11-10—Published
2010-04-23—Filed