FIELD: medicine.
SUBSTANCE: cells of bone marrow are subjected to 30-minute pre-incubation in nutrient medium which contains 1 U/ml of hyaluronidase, and are twice washed by centrifugation. After that non-adhering fraction of myelocariocytes is cultivated in nutrient medium with addition of granulocytic colony-stimulating factor.
EFFECT: invention makes it possible to perform efficient stimulation of pluripotent hemopoetic stem cells in vitro.
1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETERMINING EFFICACY OF HAEMOSTIMULATORS IN CYTOSTATIC MYELOSUPPRESSION | 2009 |
|
RU2421720C2 |
| METHOD FOR INCREASE OF STEM CELLS RESERVE IN ORGANISM | 2007 |
|
RU2347583C1 |
| HEMOSTIMULATING AGENT | 2013 |
|
RU2514648C1 |
| METHOD OF INTENSIFICATION OF STEM CELLS MOBILISATION | 2006 |
|
RU2330674C1 |
| METHOD FOR DETERMINATION OF PRODUCTION OF STEM CELL HOMING FACTORS | 2009 |
|
RU2399664C1 |
| METHOD OF MYELOPOIESIS STIMULATION | 2008 |
|
RU2366452C1 |
| METHOD FOR STIMULATING MYELOGENESIS | 2010 |
|
RU2442589C1 |
| AGENT INTENSIFYING ACTION OF BIOLOGICALLY ACTIVE SUBSTANCES AND DRUGS | 2009 |
|
RU2421239C1 |
| METHOD FOR PREPARING CELL TRANSPLANTATION MATERIAL IN MYELOSUPPRESSION | 2008 |
|
RU2392947C1 |
| METHOD OF INCREASING STEM CELLS MOBILIZATION | 2009 |
|
RU2442601C2 |
