METHOD FOR PREPARING CELL TRANSPLANTATION MATERIAL IN MYELOSUPPRESSION Russian patent published in 2010 - IPC A61K35/14 A61K38/47 A61P35/00 

FIELD: medicine.

SUBSTANCE: invention refers to experimental medicine and concerns preparation of a cell transplantation material in myelosuppression. That is ensured by subcutaneous introduction to a laboratory animal of a recombinant granulocytic colony-stimulating factor (G-CSF) in dosage 125 mcg/kg once a day for 3 days. It is combined with intraperitoneal introduction of hyaluronidase in dosage 1000 SU/kg once a day for 2 days. Then mononuclear leukocyte fraction of peripheral blood is prepared, placed in a balanced nutrient medium containing 10^-8 mol/ml of D-glucuronic acid, and incubated for 2 hours.

EFFECT: introduction of G-CSF and hyaluronidase in the developed dosage and regimen, in combination with incubation with D-glucuronic acid added provides higher therapeutic activity of the graft due to mononuclear growth in peripheral blood and better engrafting ability.

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