FIELD: biochemistry.
SUBSTANCE: method includes separate culturing of strains of lactic bacteria and bifidus bacteria on bouillon containing digest of casein produced by hydrolysis using cattle pancreas, carbohydrate component containing lactose and raffinose in ratio 1:1. Raffinose is both a carbohydrate component and probiotic component. The medium contains peptone, agar-agar, ascorbic acid and distilled water. The mixture of strains is added to sorbing component prepacked in vial in proportion of ½ - ¼ from total volume of created complex system. Process of immobilization on sorbent - Litovit M dietary supplement is performed at temperature +6°C±2°C for 18-24 hours.
EFFECT: production of immobilized synbiotic product with high content of live microbial cells, several strains of lactic bacteria and bifidus bacteria with increased protective and treatment activity.
3 cl, 2 tbl
Authors
Dates
2012-02-10—Published
2010-07-29—Filed