ANTI-GLYPICAN 3-ANTIBODY HAVING MODIFIED SUGAR CHAIN Russian patent published in 2012 - IPC C07K16/18 A61K39/395 A61P35/00 C12P21/08 

FIELD: medicine.

SUBSTANCE: there are described versions of compositions of human glypican 3 IgGl antibodies. Each composition contains a mixture of human glypican 3 antibodies in an effective amount. The mixture consists of the antibodies of standard structure and either more than 20 % of the fucose-free antibodies, or the antibodies having bisectional N-acetylglucosamide. Each antibody is characterised by a certain amino acid sequence. One version of the composition provides the antibody containing three CDR light and three CDR heavy chains. According to the other version, each antibody is characterised by the presence of variable areas of heavy and light chains. There are described versions of the method for preparing glypican 3 antibody with using a cell with the reduced ability of fucose attachment to sugar chains into which the gene coding the antibody from the composition is introduced. What is offered is an anticancer drug on the basis of the antibody composition.

EFFECT: using the invention provides higher ADCC cytotoxic activity that can find application in cancer therapy.

10 cl, 7 dwg, 2 tbl, 9 ex