FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, specifically to immunocytokines. Disclosed is an antibody and interferon α fusion protein targeting GPC3, characterized in that said fusion protein consists of an A-chain with SEQ ID NO: 1 and B-chains with SEQ ID NO: 2. Invention provides producing a fusion protein of a GPC3-targeted antibody and interferon α characterized by extremely strong inhibition of proliferation and tumour killing activity on GPC3-positive target cells (tumour cells, such as liver cancer cells HepG2 and HuH-7), safety for non-target cells that do not express GPC3, and stability during freezing and thawing, which is significantly better for the fusion protein than that of interferon α that is useful for production, transportation and application of the product.
EFFECT: invention can be used in medicine for treating or preventing a tumour associated with a positive GPC3.
9 cl, 9 dwg, 5 tbl, 8 ex
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