FIELD: process engineering.
SUBSTANCE: invention relates to production of magnetic iron-bearing nanoparticles to be used in medicine. Proposed method comprises cultivating bacteria Klebsiella oxytoca educed from sapropel of Borovoe Lake, Krasnoyarsk Region, and growing of biomass Then, centrifugation is performed to produce precipitate bearing ferrihydrite and biomass is destructed by ultrasound to isolate ferrihydrite magnetic nanoparticles. Note here that biomass cultivation and growing are executed using iron citrate for 7-10 days to produce precipitate of bacterial cultures. After biomass destruction, precipitates are centrifuged, washed by water and acetone, and treated with NaOH to produce 20%-solution. Then, hatching is performed for an hour to wash precipitates with distilled water with addition of NaCl to neutral pH. Thereafter, precipitates are isolated, washed to produce stable aqueous sol based on ferrihydrite nanoparticles and obtained sol is racked.
EFFECT: stable aqueous sol based on ferrihydrite nanoparticles.
4 dwg, 2 tbl
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Authors
Dates
2012-07-27—Published
2011-03-24—Filed