METHOD OF PRODUCING COMENIC ACID Russian patent published in 2012 - IPC A61K31/353 C12N1/20 C12R1/01 

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry and a method of producing comenic acid. The method is characterised by that it involves fermentation of the Gluconobacter oxydans-03 strain in a medium containing glucose - 35.0 g/l, yeast extract - 1.17 g/l, disubstituted ammonium phosphate - 0.619·10^-3 g/l, monosubstituted potassium phosphate - 0.12·10^-3 g/l, at temperature 28°C with forced aeration by 1.5-2.0 volume of air per 1 volume of medium per minute for 66-78 hours until obtaining gluconic acids: gluconic, 2-keto-gluconic, 5-keto-gluconic, 2,5-diketo-gluconic; Gluconobacter oxydans-03 cells are deposited by heating the culture fluid to 80-85°C for 0.5 hours in the presence of bentonite until formation of a residue; the supernatant fluid is filtered; the obtained filtrate which contains comenic acid and gluconic acids is deposited on anionite AV-17-8 chS, while adding sodium bicarbonate to obtain sodium salts of comenic acid and gluconic acids; 17-19% hydrochloric acid solution is added to form a residue containing comenic and gluconic acids; comenic acid is separated from gluconic acids by washing the residue with iced water or iced water-alcohol solution with water to alcohol ratio of 3:1, wherein 10 ml of the solution per 1 g of residue is used.

EFFECT: invention enables to obtain high-purity comenic acid.

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