FIELD: medicine.
SUBSTANCE: what is presented is a method for preparing the substance L-lysine-alpha-oxydase (LO) with the use of the Trichoderma cf. aureoviride Rifai BKMF-4268D producer strain. It is followed by fermentation on a medium containing nitrogen, phosphate sources and wheat bran. It is followed by recovery and purification of the enzyme in the following sequence of the procedures: culture fluid dialysis, adsorbent treatment, impurity sedimentation in 20%-saturated ammonium, hydrophobic and ion-exchange chromatography. It is followed by preparing L-lysine-alpha-oxydase of molecular weight 115-116 kDa, isoelectric point 4.25, optimum pH - 7.4.
EFFECT: higher yield of LO.
5 tbl, 5 ex
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