FIELD: medicine.
SUBSTANCE: there are presented specific oligonucleotide primers for identifying a variable DNA fragment of the equinia agent strains possessing activity in an amplification reaction having the following structure: 5' - CCG TTG TCC TTC GTG TTC AT - 3' - BmVAT5-Ch2s 5' - AGC ACA CAT TCG CCG TCC T - 3' - BmVAT5-Ch2as. The invention may be used in medicine for detection of the differentiation DNA fragment BMAA0107 comprised of a genotyping diagram of the equinia agent Burkholderia mallei both for practical healthcare, Federal Service on Surveillance for Consumer rights protection and human well-being, and for research investigations.
EFFECT: higher primer activity.
3 dwg, 3 ex
Title | Year | Author | Number |
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OLIGONUCLEOTIDE PRIMERS FOR GENETIC TYPING B. mallei BY POLYMERASE CHAIN REACTION | 2012 |
|
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SET OF FLUORESCENTLY LABELLED OLIGONUCLEOTIDE PROBES FOR TYPING STRAINS OF Burkholderia mallei BY METHOD OF AMPLIFICATION OF DIFFERENTIATING DNA FRAGMENTS | 2014 |
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RU2551227C1 |
OLIGONUCLEOTIDE PRIMERS BURK1526S/BURK1526AS FOR ASSESSMENT OF ADAPTIVE VARIABILITY OF GENOME OF PATHOGENIC BURKHOLDERIA | 2013 |
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RU2545710C2 |
Authors
Dates
2013-02-10—Published
2012-01-26—Filed