FIELD: biotechnology.
SUBSTANCE: probes included in the set have a structure of a "pin" with fluorophore and fluorescence extinguisher at the ends and have complementarity to products of an amplification reaction with primers. The developed set of hybridisation probes can be used for the fluorescent detection of the results of typing the actinobacillus mallei by a method of amplification of differentiating fragments of a genome in the implementation of the epidemiological surveillance of the glanderous infection.
EFFECT: possibility of simultaneous analysis of nine DFR-loci.
2 dwg, 1 tbl, 3 ex
Title | Year | Author | Number |
---|---|---|---|
OLIGONUCLEOTIDE PRIMERS FOR GENETIC TYPING B. mallei BY POLYMERASE CHAIN REACTION | 2012 |
|
RU2474615C1 |
OLIGONUCLEOTIDE PRIMERS FOR GENETIC TYPING B. mallei BY POLYMERASE CHAIN REACTION | 2012 |
|
RU2474618C1 |
OLIGONUCLEOTIDE PRIMERS FOR B.mallei GENETIC TYPING BY POLYMERASE CHAIN REACTION | 2012 |
|
RU2478714C1 |
OLIGONUCLEOTIDE PRIMERS FOR GENETIC TYPING B. mallei BY POLYMERASE CHAIN REACTION | 2012 |
|
RU2474616C1 |
OLIGONUCLEOTIDE PRIMERS FOR GENETIC TYPING B. mallei BY POLYMERASE CHAIN REACTION | 2012 |
|
RU2474619C1 |
OLIGONUCLEOTIDE PRIMERS BmVAT5-Ch2s/BmVAT5-Ch2as FOR DETECTING BMAA0107 DIFFERENTIATION FRAGMENT OF EQUINIA AGENT STRAINS | 2012 |
|
RU2474620C1 |
OLIGONUCLEOTIDE PRIMERS FOR GENETIC TYPING B. mallei BY POLYMERASE CHAIN REACTION | 2012 |
|
RU2474617C1 |
OLIGONUCLEOTIDE PRIMERS FOR B.mallei GENETIC TYPING BY POLYMERASE CHAIN REACTION | 2012 |
|
RU2478715C1 |
OLIGONUCLEOTIDE PRIMERS BmVAT7-Ch2s/BmVAT7-Ch2as FOR DETECTING DIFFERENTIATION FRAGMENT BMAA0871 OF EQUINIA AGENT STRAINS | 2012 |
|
RU2478713C1 |
SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES AND METHOD FOR DETECTING DNA OF AGENTS OF GLANDERS AND MELIOIDOSIS BY PCR METHOD WITH PRODUCT DETECTION IN REAL TIME | 2019 |
|
RU2738358C1 |
Authors
Dates
2015-05-20—Published
2014-03-04—Filed