METHOD FOR BIOREMEDIATION OF WATER CONTAMINATED WITH TRINITROTOLUENE Russian patent published in 2013 - IPC C02F3/34 C12N1/16 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. Disclosed is a method for bioremediatio of water contaminated with 2,4,6-trinitrotoluene (TNT). The method is carried out in three series-connected bioreactors with continuous flow of the purified medium. At the first step, bioreactors are filled by a third with a liquid TNT-containing medium and mixers and the aeration system are turned on. A day old culture of yeast Yarrowia lipolytica VKPM Y-3492 is then added to the first bioreactor. The Y. lipolytica is cultured until full conversion of TNT to a S-3 monohydride Meisenheimer complex of a deep red colour. A day old culture of Y. lipolytica is added to the second bioreactor at the same time as the beginning of operation of the first bioreactor and then cultured until accumulation of yellow-orange S3, S5 dihydride Meisenheimer complexes. A day old culture of Y. lipolytica is added to the third bioreactor at the same time as the beginning of operation of the first and second bioreactors and then cultured until full decomposition of TNT-dihydride complexes with decolouration of the purified medium. At the second step, the biodegradation of TNT is carried out in continuous mode of culturing Y. lipolytica. The process of culturing Yarrowia lipolytica VKPM Y-3492 is carried out at temperature ranging from +24°C to +31°C, pH from 3.0 to 7.0 and rate of mixing the medium of 100-250 rpm. The source of carbon and energy for growth of the strain and degradation of TNT used is monosaccharides or triatomic alcohols or aliphatic hydrocarbons.

EFFECT: method increases efficiency of the process of decontaminating water contaminated with TNT and cuts the bioremediation time: provides 85% decomposition of TNT in concentration of 100 ml/g a day.

1 dwg, 3 ex