SINGLE-CHAIN MULTIVALENT BINDING PROTEINS WITH EFFECTOR FUNCTION Russian patent published in 2013 - IPC C07K16/28 C07K16/46 C12N15/13 C12N15/63 A61K39/395 A61P35/00 A61P37/00 

FIELD: chemistry.

SUBSTANCE: disclosed is a single-chain multi-specific binding protein for binding or modulating activity of one or more biding partner molecules. The protein contains from the N-terminal to the C-terminal: a first binding domain derived from variable regions of a light and a heavy immunoglobulin chain; a constant sub-region derived from immunoglobulin containing a hinge or hinge-like domain, a C_H2-domain and a C_H3-domain; scorpion linker peptide, where said peptide contains an amino acid sequence from the immunoglobulin hinge or a type II C-lectin protein stalk region; and a second binding domain derived from variable regions of a light and a heavy immunoglobulin chain. The invention describes an encoding nucleic acid, as well as an expression vector based thereon and a host cell for vector based protein expression. The invention discloses a pharmaceutical composition for treating cell proliferation disorders based on a single-chain protein and a protein dimer for binding or modulating activity of one or more binding partner molecules based on two single-chain binding proteins.

EFFECT: invention provides multi-specific molecules which retain effector functions, insensitivity to proteolytic cleavage, and can be used in therapy of different diseases.

31 cl, 61 dwg, 14 tbl, 19 ex