FIELD: chemistry.
SUBSTANCE: invention relates to field of biotechnology and virology. Method of selecting human influenza virus for growing on tissue culture cells by method of limiting dilution cloning. Method includes dilution of influenza virus, its mixing with tripsin, contact with cells of tissue cultures, growing and harvesting of virus. Said stages are performed 2-3 times. Also described is method of obtaining vaccine against human influenza virus with application of claimed method of selecting influenza virus.
EFFECT: method makes it possible to cultivate influenza virus of cell cultures.
16 cl, 2 dwg, 5 tbl, 11 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR INITIAL ISOLATION OF INFLUENZA VIRUS A STRAIN, STRAIN VIRUS A/DUCK/NOVOSIBIRSK/56/05 H5N1 FOR PREPARATION FOR DIAGNOSIS, PROPHYLAXIS AND THERAPEUTICAL AGENTS, AND FOR EVALUATION OF ANTIVIRAL ACTIVITY OF VARIOUS COMPOUNDS | 2005 |
|
RU2309983C2 |
METHOD OF PRODUCTION OF LIVE CULTURE INFLUENZA VIRUS VACCINES | 2003 |
|
RU2330885C2 |
INFLUENZA VACCINE | 2011 |
|
RU2583297C2 |
INFLUENZA INFECTING DOGS AND USE THEREOF | 2014 |
|
RU2711807C2 |
FLU VIRUS, CAPABLE OF INFECTING CANIDAE AND ITS APPLICATION | 2006 |
|
RU2520081C2 |
ANTIVIRAL AGENT | 2014 |
|
RU2564919C1 |
VACCINES AND METHODS OF TREATING CANINE INFLUENZA | 2006 |
|
RU2396976C2 |
METHOD FOR PRODUCING REPLICATIVE INFLUENZA VIRUS PARTICLES, CELL COMPOSITION (VERSIONS), CELL CULTURE COMPOSITION AND APPLICATION THEREOF | 2005 |
|
RU2435855C2 |
RECOMBINANT INFLUENZA VIRUS STRAINS EXPRESSING MYCOBACTERIAL PROTECTIVE ESAT-6 ANTIGEN AND USES THEREOF IN TUBERCULOSIS TREATMENT AND PROPHYLAXIS | 2005 |
|
RU2318872C2 |
MODIFIED VARIANT OF ANKARA VACCINA VIRUS | 2001 |
|
RU2290438C2 |
Authors
Dates
2013-08-27—Published
2007-12-14—Filed