FIELD: chemistry.
SUBSTANCE: group of inventions relates to composition of an analysing sensor reagent, which is adapted to facilitate determination of concentration of an analyte in a liquid sample, methods of determining concentration of an analyte in a liquid sample and a method of depositing the composition of the analysing sensor reagent onto a substrate by screen printing. The composition of the analysing sensor reagent contains 1-4.0 wt % glucose oxidase enzyme, 15-20 wt % ferricyanide mediator, 3.6-6.0 wt % hydroxyethyl cellulose polymer and 0.2-1.6 wt % smectite clay. The method of determining concentration of an analyte in a liquid sample involves making an electrochemical analysing sensor which consists of counter electrode and a working electrode, a region for receiving liquid and said analysing sensor reagent, and determining concentration of the analyte in less 35 s. Another method of determining concentration of an analyte in a liquid sample involves pricking the finger of the human subject in order to collect a liquid sample, placing the liquid sample containing one analyte into the analysing sensor, bringing the liquid sample into contact with the analysing sensor reagent and determining concentration of the analyte in the liquid sample. The method of depositing said composition of an analysing sensor reagent onto a substrate by screen printing involves making a mesh screen consisting of a first part with a light-sensitive emulsion and a second part without a light-sensitive emulsion; feeding the analysing sensor reagent onto the mesh screen, and bringing the reagent into contact with the substrate through the second part of the mesh screen.
EFFECT: high sensor stability, easy application of the reagent, shorter overall duration of analysis and high adhesion of the reagent to the substrate.
14 cl, 11 dwg, 5 ex
