METHOD OF ASSESSMENT OF GENE EXPRESSION OF TRYPTOPHANYL - TOTAL RNA SYNTHETASE AS MARKER OF OVERWORK DURING TRAINING OF ATHLETES - STATE OF OVERTRAINING Russian patent published in 2014 - IPC C12Q1/68 C12N9/00 G01N33/50 

FIELD: biotechnology.

SUBSTANCE: invention is a method of determining in an athlete of state of fatigue and a state of "overtraining" on increased gene expression of tryptophanyl-total RNA synthetase (TRSase). The method comprises taking a control sample from the athlete prior to intense training and the test sample after an intense training. As the sample the sample of blood or scraping or flushing with the oral cavity is used. The obtained cells are selected and washed. Total RNA is isolated from the cells. Reverse transcription is carried out and then amplifying of the cDNA obtained. The gene expression in TRSase is assessed in test and the control samples. Fatigue state and the state of "overtraining" is determined in case of a significant increase in gene expression level of TRSase in the test sample compared with a control sample, namely more than 1.45 times.

EFFECT: invention enables to increase significantly informational content, to simplify and accelerate the procedure of testing.

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