FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology. Disclosed is a method of producing millerite by placing a pure culture of sulphate-reducing bacteria, which are resistant to copper ions and ions of other metals, into a synthetic medium containing metal salts, with addition of divalent nickel and nutrients, which include solutions of vitamins, potassium, ammonium, sodium and calcium salts, cofactors, lactate and sodium sulphate. Glycerine is added to the culture medium. Bacteria are cultured at temperature of 28°C. The formed precipitate, which contains millerite, is collected by centrifuging and then dried. Formation of crystalline millerite begins in 7 days and a stable crystalline millerite phase forms in 20 days.
EFFECT: method enables to obtain millerite which does not contain impurities of other sulphides in short culturing periods.
6 dwg, 3 tbl, 1 ex
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Authors
Dates
2014-09-20—Published
2012-10-05—Filed