FIELD: chemistry.
SUBSTANCE: incubation medium, oxidation substrate and tetraphenylphpsphonium chloride as indicator are placed into respective measuring cell of installation for measuring mitochondria potential, provided with tetraphenylphosphonium-selective electrode, change of tetraphenylphosphonium concentration is registered and when constant tetraphenylphosphonium concentration is achieved, mitochondria, isolated from animal organism, are added. Change of membrane potential of mitochondria is registered by change of electrode signal, when constant potential is achieved, respective water suspensions of analysed samples of nanodiamonds with pH 7.2-7.4 are added, and value of rate of change of mitochondria membrane potential is measured. Presence of statistically reliable difference of rates of mitochondria membrane potential change testifies to biological inequivalence of compared samples of nanodiamonds.
EFFECT: expressive and available method of determining biological inequivalence of nanodiamonds.
2 cl, 1 tbl, 1 dwg, 1 ex
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