CONJUGATE OF NANODIAMOND WITH PYROPHOSPHATASE AND METHOD OF OBTAINING THEREOF Russian patent published in 2015 - IPC A61K38/46 A61K47/02 A61K47/48 C01B31/06 B82Y5/00 

FIELD: chemistry.

SUBSTANCE: conjugate represents nanodiamond particles with size 2-10 nm with pyrophosphorase, immobilised on them by means of linker, containing amino or amide groups. Content of pyrophosphatase constitutes 0.1-1 mg per 1 mg of nanodiamond, with specific activity of pyrophosphatase constituting to 95±5% of native pyrophosphatase activity. method of conjugate obtaining includes dissolution of nanodiamond with grafted hexamethylenediamine and/or nanodiamond aminated with ammonia in water, successive addition of water buffer solution HEPES with pH 7-8, magnesium chloride, sodium fluoride, sodium pyrophosphate, pyriphosphatase, and glutaraldehyde. After that, obtained mixture is exposed for 0.5-12 h, centrifuged, washed with water buffer solution Tris-HCl and dried.

EFFECT: obtaining conjugate of nanodiamond with pyrophosphatase, possessing increased stability.

3 cl, 1 tbl, 2 dwg, 2 ex