FIELD: biotechnologies.
SUBSTANCE: invention refers to a method of preliminary protein sample preparation for electrophoresis, involving meat and meat product mincing, homogenization, homogenate centrifuging at 20°C for 30 minutes with further storage of samples obtained, where homogenization is performed with 10% saccharose solution, centrifuging is performed at 10000 rpm rate, and samples are stored at -4±2°C.
EFFECT: simplified protein sample preparation.
2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
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