CD3-EPSILON-BINDING DOMAIN HAVING INTERSPECIES SPECIFICITY Russian patent published in 2015 - IPC C07K16/28 C07K16/46 C12N5/10 C12N15/13 C12N15/63 A61K39/395 A61P35/00 A61P37/00 

FIELD: medicine.

SUBSTANCE: present invention refers to biotechnology and immunology. What is presented is a polypeptide, which represents a bispecific single-chain antibody binding to human and primate Callithrix jacchus, Saguinus Oedipus and Saimiri sciureus CD3ε(epsilon)-chain epitope containing the sequence Gln-Asp-Gly-Asn-Glu (QDGNE); there are also presented a polypeptide binding a domain representing an antibody binding to human and primate Callithrix jacchus, Saguinus Oedipus and Saimiri sciureus CD3ε(epsilon)-chain epitope, and a second binding domain representing an antibody representing an antibody binding to a cell surface antigen. There are also disclosed nucleic acid sequences coding the polypeptides according to the invention, vectors, a host cell, a method based on using the same and applicable for producing the polypeptides according to the invention, a pharmaceutical composition and using the polypeptides according to the invention for preventing, treating or relieving a disease specified in a proliferative disease, a tumour disease or an immune disorder, as well as using an N-terminal fragment of CD3ε outer domain for producing the polypeptide according to the invention.

EFFECT: modulating the T-cell immunity in relation to the cells expressing prostate-specific membrane antigen.

22 cl, 68 dwg, 16 tbl, 41 ex