FIELD: medicine.
SUBSTANCE: invention relates to veterinary, in particular to aspects, associated with cellular immunity, and describes a method for the reduction of the hormonally conditioned immunosuppressive effect of cellular immunity with homoeopathic medications in vitro. The method is characterised by the fact that the hormonal medication glycortin-20 is added to a leukocytic suspension, with the further introduction after incubation of a homoeopathic medication, diluted in physiological solution in a selected from Phytolac C6 or C200, Arsenicum album C6 or C12 or C30 or C200, Lachesis D15 or C12 or C30 or C200, Aconite C6 or C12 or C200, Belladonna C30, Nux vomica C6, Phenogrossi C6 or C12, Conium C30, Arnica C12, Ipecacuanha C6 or C200 dilution, then after incubation introduced is a suspension of microorganisms of a reference strain of Staphylococcus albus No 182, which contains 109 CFU/ in 1 ml of physiological solution, then after incubation smears of blood are fixed in alcohol-formalin, taken in a ratio of 1:9, which contains 40% formalin solution and 96° alcohol for 10 minutes, and then stained by Romanowski-Giemsa for 30 minutes.
EFFECT: invention can be applied in the veterinary practice for the removal of the immunosuppressive effect of hormonal steroid medications in the research of cellular immunity in animals.
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METHOD FOR CELL IMMUNITY ACTIVATION in vitro WITH HOMEOPATHIC MEDICINES | 2013 |
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Authors
Dates
2015-09-20—Published
2013-11-18—Filed