METHOD OF REDUCING TOXIC EFFECT OF ENROFLOXACIN ON CELLULAR IMMUNITY WITH APPLICATION OF HOMEOPATHIC PREPARATIONS in vitro Russian patent published in 2015 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: invention relates to veterinary and can be applied for reduction of toxic effect of enrofloxacin on opsonophagocytic activity of neutrophils with application of homeopathic preparations in vitro. For this purpose 0.15 ml of antibacterial preparation enrofloxacin 5% are added to leukocyte suspension in volume 0,5 ml. After that obtained solution is placed into thermostat for 3 hours at T=37±0.5°C, after incubation 0.5 ml of homeopathic preparation, diluted in physiological solution in selected dilution C6, C12, C30, C200. After that test-tubes are placed back into thermostat for 4 hours at T=37±0.5°C, after which suspension of microorganisms of reference strain Staphylococcus albus No 182 is introduced into each test-tube in dose 0.5 ml, containing 108 CFU in 1 ml of physiological solution, with the following placement into thermostat for 30 min at T=37±0.5°C, after incubation blood smears are fixed in alcohol-formalin 1:9, 40% solution of formalin and 96° alcohol, for 10 minutes and Romanowsky-Giemsa stained for 30 minutes.

EFFECT: application of claimed method makes it possible to estimate in vitro impact of homeopathic preparations on reduction of toxic effect of enrofloxacin on opsophasocytic activity of neutrophils.

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