FIELD: chemistry.
SUBSTANCE: invention relates to the field of biochemistry, in particular to a method of obtaining muteins of human lipocalin 2 (Lcn2, hNGAL). The method is based on the mutagenesis of a molecule of a nucleic acid, coding the human Lcn2 protein. At least a nucleotide triplet, coding position 96 in a linear polypeptide sequence of the human Lcn2 is subjected to the mutagenesis. The muteins, obtained by the claimed method, which bind with a preliminarily specified target with identifiable affinity, where the said target does not bind with natural hGAL under physiological conditions are also claimed.
EFFECT: invention relates to molecules of nucleic acid, which code such muteins, a host cell, as well as to diagnostic and analytical sets, including the muteins in accordance with the invention.
20 cl, 28 dwg, 3 tbl, 24 ex
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