NOVEL SPECIFIC BINDING POLYPEPTIDES AND ROUTES OF USE THEREOF Russian patent published in 2020 - IPC C07K14/47 G01N33/68 C12P21/02 A61K38/00 A61P29/00 

FIELD: biotechnology.

SUBSTANCE: present invention relates to novel polypeptides of mutein of lipocalin, and can be used for binding IL-17A for the purpose of detection of its presence or inhibition of IL-17A binding with its receptor IL-17RA. Lipocalin mutein comprising mutations is obtained Arg 26 → Phe; Glu 27 → Trp; Phe 28 → Cys; Pro 29 → Ser; Glu 30 → Gly; Met 31 → Ile; Asn 32 → His; Leu 33 → Glu; Leu 56 → Asp; Ser 58 → Glu; Arg 60 → Phe; Cys 61 → Leu; Val 64 → Phe; His 92 → Arg; Cys 101 → Ser; Glu 104 → Asp; Leu 105 → Cys; His 106 → Pro; deletion of Lys 108; Arg 111 → Pro; Lys 114 → Trp and Cys 153 → Ser as compared to a linear polypeptide sequence of mature human tear lipocalin with SEQ ID NO: 41.

EFFECT: invention enables to obtain an IL-17A-affinity mutein of lipocalin.

48 cl, 5 tbl, 19 ex, 19 dwg