IMPROVED PROTEIN PURIFICATION BY MEANS OF MODIFIED ELUATING OF PROTEIN A Russian patent published in 2015 - IPC C12P21/00 C07K1/22 

FIELD: biotechnologies.

SUBSTANCE: invention relates to the method of purification of the polypeptide containing C_h2/C_h3-region. The method includes linking of polypeptide with protein A and eluating of polypeptide with pH gradient starting from 5.0 or less using the eluating buffer, and the eluating buffer includes the buffer with high pH level and the buffer with low pH level, and pH gradient is formed as a result of regulation of percentage of each pH-buffer in the eluating buffer. The buffer with high pH level has pH about 5.0. The buffer with low pH level has pH about 2.7.

EFFECT: invention allows to separate the aggregate, admixture formed by host cells, pollution of the virus filter, separate the virus particle or virus-like particle from the required polypeptide.

21 cl, 21 dwg, 8 tbl, 11 ex