FIELD: biotechnology.
SUBSTANCE: present invention provides methods for production of heteromultimeric proteins with the ability to specifically bind to more than one target (versions), as well as methods for creation of combinatorial libraries of such heteromultimeric proteins (versions). The described methods comprise culturing of the first host cell expressing the first heavy and light chains, and the second host cell expressing the second heavy and light chains, obtaining of a combined culture containing the first and the second host cells to form a heteromultimeric protein.
EFFECT: this invention allows efficient and high level genes expression and simple assembly of heteromultimeric proteins.
52 cl, 49 dwg, 4 tbl, 7 ex
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