METHOD OF DETERMINING CONTENT OF CADMIUM, LEAD, ARSENIC, CHROMIUM, NICKEL, COPPER, ZINC, MANGANESE, VANADIUM, STRONTIUM, SELENIUM, THALLIUM IN BLOOD BY MASS SPECTROMETRY WITH INDUCTIVELY COUPLED PLASMA Russian patent published in 2016 - IPC G01N33/84 

FIELD: chemistry.

SUBSTANCE: invention relates to analytical chemistry and can be used in sanitary-hygienic, ecological, medical and research organisations, operating in area of occupational pathology and human ecology. Summary: blood is sampled and prepared by adding to sample concentrated nitric acid, heating mixture and its dilution with deionised water with subsequent introduction of prepared sample into sampling device of mass-spectrometer with inductively coupled plasma, performance of measurements and determination of content of specific metal using calibration curve. Prior to adding to blood sample with concentrated nitric acid with density of 1.415 g/cm³ in sample is introduced complex internal standard solution of terbium, indium and germanium in deionised water with weight concentration of each of said element 100 mcg/dm³ in volume ratio of above complex solution to blood sample as 1:1 or 1:2 and in volume ratio of blood sample to concentrated nitric acid as 1:2. Mixture of sample with the above complex internal standard solution and with concentrated nitric acid is heated in water bath at a temperature of 65-70 °C to homogenisation. Further dilution of obtained mixture with deionised water is performed by introducing latter in volume of sum with volume of mixture of 100 vol parts and before prepared sample into sampler of mass spectrometer is centrifuged for 10 minutes at 2,700-3,000 rpm. At that, performance of measurements in prepared sample content of specific metal in mass spectrometer is carried out using reactive/collisional cell at passage through it of helium at a rate of 4.5-5.0 cm³/min. With specified measurement of content of lead and thallium in internal standard used is terbium, when determining cadmium are indium or terbium, and determination of other metals is germanium.

EFFECT: high sensitivity and accuracy of analysis while simultaneously reducing amount of blood sample required for analysis.

1 cl, 4 dwg, 11 tbl