FIELD: biotechnology.
SUBSTANCE: invention relates to a set of oligonucleotide primers for obtaining a primary structure F of Newcastle disease virus gene class I. Presented set consists of three pairs of oligonucleotides having following structure (5′→3′):
Fgene 1F - ATGGGCTCCAGATCTTCTACC, Fgene 560R
TTAACAAATTGYTGCATCTTCCCG;
Fgene 471F - GCATTGCTGCAACCAATGAGG, Fgene 1008R
TGAGGTGTCAAGYTCTTCTATCAC;
Fgene 898F - CGTGCCACCTACYTGGAAAC, Fgene 1663R
TCACATTTTYGTAGTGGCYCTC. Presented oligonucleotides avoid cross-reactions with related species, make it possible to limit manipulation of single-stage procedure of polymerase chain reaction (PCR). Developed primers enable to obtain full information on F genes of Newcastle disease virus, their belonging to any genetic line virus, presence of nucleotide/amino acid substitutes in genome of pathogen.
EFFECT: invention can be used for diagnostic purposes for identification of Newcastle disease virus in virology and veterinary science, as well as to solve scientific and research tasks on study of said virus.
1 cl, 1 dwg, 2 tbl, 3 ex
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