METHOD OF CARDIOPROTECTIVE ACTIVITY ESTIMATION OF PHARMACOLOGICAL FACILITIES Russian patent published in 2018 - IPC A61B5/21 G09B23/28 

FIELD: medicine; pharmacology.

SUBSTANCE: invention relates to medicine, namely, to experimental cardiac pharmacology, and can be used in a comprehensive assessment of the activity of pharmacological agents on an isolated rat heart. For this, method includes the registration of indices of contractility under conditions of imposing a high rhythm of contractions and hypercalcium load on the heart. At the same time, the parameters of the contractile function of the left ventricle are recorded, then a 20-minute perfusion of the heart with a solution containing 5 mmol/l of Ca²⁺ is performed to determine the dynamics of the diastolic stress. In the future, the reduction frequency is set to 480 bpm for 15 seconds using an electric stimulator. Then the coefficient S_TTI (area of defect diastole) is calculated in conventional units by folding trapezium squares under the curve of elevation of the end-diastolic pressure and evaluating the contractile function of the heart, taking into account the physiological values of S_TTI.

EFFECT: method provides information and increase in the reliability of evaluation indicators of the degree of cardioprotective or cardiotoxic activity pharmacological means using the coefficient S_TTI.

1 cl, 2 dwg, 1 ex, 3 tbl