LINE OF BIOLOGICALLY ACTIVE GENE-THERAPEUTIC SUBSTANCES BASED ON THE GENE OF GPX1 FOR CORRECTION OF THE PATHOLOGICAL CONDITIONS OF THE CELLS OF ORGANS AND TISSUES AND HUMAN ORGANS AND TISSUES RELATED TO THE OXIDATIVE STRESS, THE METHOD OF OBTAINING AND USING Russian patent published in 2018 - IPC C12N15/00 A61P39/06 

Abstract RU 2653491 C2

FIELD: medicine.

SUBSTANCE: group of inventions refers to medicine and concerns a means for correcting the pathological conditions of cells of organs and tissues and/or human organs and tissues based on the GPX1 gene, where the cells of organs and tissues are selected from fibroblasts, keratocytes and epithelial cells of the eye, chondroblasts; organs and tissues are selected from the skin, mucous membrane of the mouth or muscle tissue, which is a combination of gene therapy substances, each of which represents a gene therapy substance selected from a group of genetic therapeutic substances, each being a genetic construct based on a vector plasmid comprising the cDNA of the GPX1 gene, with the glutathione peroxidase-1 protein coding sequence, with deletions of 5' and 3' non-translated regions, namely obtained from the site of the native unmodified cDNA of the GPX1 gene SEQ ID No: 1, or a modified cDNA of the GPX1 gene, wherein as the modified cDNA of the GPX1 gene, SEQ ID No: 2, or SEQ ID No: 3, or SEQ ID No: 4, or SEQ ID No: 5, or SEQ ID No: 6, or SEQ ID No: 7, or a combination of these genetic constructs, each of which also contains regulatory elements that ensure transcription of this sequence in human cells and tissues, in combination with or without a transport molecule. Group of inventions also relates to a method for producing said agent; a method of using said agent for correcting the pathological conditions of cells of organs and tissues and/or human organs and tissues based on the GPX1 gene.

EFFECT: group of inventions provides a high and stable level of the target protein in the cells.

5 cl, 18 ex, 20 dwg

Similar patents RU2653491C2

Title Year Author Number
MEANS FOR CORRECTION OF PATHOLOGICAL CONDITIONS OF CELLS OF ORGANS AND TISSUES AND/OR HUMAN ORGANS AND TISSUES, BASED ON THE GENE OF GPX3, RELATED TO OXIDATIVE STRESS, METHOD OF OBTAINING AND USING 2016
  • Savelieva Natalia
  • Gamolski Anton
RU2651758C2
LINE OF BIOLOGICALLY ACTIVE GENE-THERAPY SUBSTANCES BASED ON SOD1 GENE FOR CORRECTION OF PATHOLOGICAL STATES OF CELLS OF ORGANS AND TISSUES AND HUMAN ORGANS AND TISSUES RELATED TO OXIDATIVE STRESS, METHOD OF OBTAINING AND USING 2016
  • Savelieva Natalia
  • Gamolski Anton
RU2652353C2
LINE OF BIOLOGICALLY ACTIVE GENE-THERAPEUTIC SUBSTANCES BASED ON SOD2 GENE FOR CORRECTION OF PATHOLOGICAL CONDITIONS OF CELLS OF ORGANS AND TISSUES AND HUMAN ORGANS AND TISSUES RELATED TO OXIDATIVE STRESS, METHOD OF OBTAINING AND USING 2016
  • Savelieva Natalia
  • Gamolski Anton
RU2651757C2
LINE OF BIOLOGICALLY ACTIVE GENE-THERAPY SUBSTANCES BASED ON TGFB1 GENE FOR CORRECTION OF PATHOLOGICAL STATES OF CELLS OF ORGANS AND TISSUES AND HUMAN ORGANS AND TISSUES, METHOD OF OBTAINING AND USING 2016
  • Savelieva Natalia
  • Gamolski Anton
RU2652312C2
LINE OF BIOLOGICALLY ACTIVE GENE-THERAPY SUBSTANCES BASED ON TGFBR2 GENE FOR CORRECTION OF PATHOLOGICAL STATES OF CELLS OF ORGANS AND TISSUES AND HUMAN ORGANS AND TISSUES, METHOD OF OBTAINING AND USING 2016
  • Savelieva Natalia
  • Gamolski Anton
RU2652351C2
LINE OF BIOLOGICALLY ACTIVE GENE THERAPY SUBSTANCES BASED ON COL1A1 GENE FOR CORRECTION OF PATHOLOGICAL STATES OF CELLS OF ORGANS AND TISSUES AND HUMAN ORGANS AND TISSUES, METHOD OF OBTAINING AND USING 2016
  • Savelieva Natalia
  • Gamolski Anton
RU2652350C2
AGENT FOR CORRECTION OF PATHOLOGICAL STATES OF CELLS AND TISSUES AND/OR ORGANS AND HUMAN TISSUE BASED ON COL1A2 GENE, RELATED TO QUANTITATIVE DECREASE OF PROTEIN OF COLLAGEN TYPE I ALPHA 2 CHAIN 2016
  • Savelieva Natalia
  • Gamolski Anton
RU2649820C2
LINE OF BIOLOGICALLY ACTIVE GENE-THERAPY SUBSTANCES BASED ON CLCA2 GENE FOR CORRECTION OF PATHOLOGICAL STATES OF CELLS OF ORGANS AND TISSUES AND HUMAN ORGANS AND TISSUES, METHOD OF OBTAINING AND USING 2016
  • Savelieva Natalia
  • Gamolski Anton
RU2652318C2
MEANS FOR TREATMENT OF HUMAN BODY STATES RELATED TO DECREASE OF THE CAT GENE EXPRESSION LEVEL AND/OR REDUCTION OF THE CATALYSIS PROTEIN ACTIVITY BASED ON GENE-THERAPEUTIC SUBSTANCES WITH THE CAT GENE, METHOD FOR PRODUCTION AND USE 2017
  • Savelieva Natalia
  • Sadykova Galina Kadymovna
  • Savelieva Natalia
RU2649814C1
AGENT FOR TREATMENT OF HUMAN BODY CONDITIONS ASSOCIATED WITH A DECREASE IN THE LEVEL OF ANG GENE EXPRESSION AND/OR A REDUCTION IN THE AMOUNT AND/OR ACTIVITY OF AN ANGIOGENIN PROTEIN BASED ON GENE THERAPY, A METHOD OF PRODUCTION AND USE 2017
  • Savelieva Natalia
  • Savelieva Natalia
RU2665771C1

RU 2 653 491 C2

Authors

Savelieva Natalia

Gamolski Anton

Dates

2018-05-08Published

2016-01-20Filed