FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and describes a method for non-invasive diagnosis of foetal aneuploidy by analysing extracellular DNA from the blood of a pregnant woman by mass parallel DNA sequencing. Described method is characterized by a new method for sample preparation of extracellular DNA from blood samples. This method can be used in clinical diagnostic laboratories of medical institutions.
EFFECT: technical result of the present invention is an increase in the efficiency of the process of determining foetal aneuploidy, which is achieved by the fact that per reading of the formed DNA libraries potentially obtaining information on several different chromosomes and using this information to determine foetal aneuploidy.
4 cl, 9 dwg, 1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR NONINVASIVE PRENATAL DIAGNOSTICS OF FETAL ANEUPLOIDY | 2015 |
|
RU2627673C2 |
| NON-INVASIVE PRENATAL DIAGNOSIS OF FOETAL ANEUPLOIDY | 2014 |
|
RU2583830C2 |
| METHOD FOR IDENTIFYING FETAL ANEUPLOIDY IN A BLOOD SAMPLE OF THE PREGNANT WOMAN | 2021 |
|
RU2777072C1 |
| METHOD FOR NON-INVASIVE PRENATAL SCREENING OF FETAL ANEUPLOIDY | 2019 |
|
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| METHODS AND SYSTEMS FOR DETERMINING WHETHER GENOME IS ABNORMAL | 2011 |
|
RU2599419C2 |
| METHOD FOR NON-INVASIVE PRENATAL DIAGNOSTICS OF TRISOMY AND A SET FOR IT | 2020 |
|
RU2734484C1 |
| METHOD AND APPARATUS FOR DETERMINING FRACTION OF EXTRACELLULAR NUCLEIC ACIDS IN BIOLOGICAL SAMPLE AND USE THEREOF | 2015 |
|
RU2699728C2 |
| NON-INVASIVE DIAGNOSTIC TECHNIQUE FOR FOETAL ANEUPLOIDY BY SEQUENCE ANALYSIS | 2014 |
|
RU2543155C1 |
| METHOD OF DETERMINING THE SOURCE OF ANEUPLOID CELLS ON THE BLOOD OF A PREGNANT WOMAN | 2016 |
|
RU2674700C2 |
| DIAGNOSTIC TECHNIQUE FOR ANEUPLOIDY BY SEQUENCE ANALYSIS | 2012 |
|
RU2529784C2 |
