CONTROLLED VACUUM DEGASSING OF GAS FILTER FOR FLUID MEDIUM TREATMENT SYSTEM Russian patent published in 2020 - IPC C12M1/00 C12M1/02 C12M1/34 C12Q1/16 

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to systems for treating a fluid medium in which cells or microorganisms are grown, and associated gas filtration systems which create a partial vacuum on the downstream side of the gas filter. Gas filtration system comprises a container having a compartment configured to hold the liquid medium. Liquid medium is located in the compartment and contains a culture containing a medium with suspended cells or microorganisms. In the lower part of the container there is a bubbler for gas delivery into the liquid medium inside the container, and the first filtration unit. First filtration unit comprises first gas filter. First filtration unit is connected to the container so that gas can pass from the container compartment to the first gas filter. Gas filtration system also comprises the first vacuum pump. Latter is connected to the first filtration unit so that with the first vacuum pump actuated, a partial vacuum is created, which facilitates gas passage from the container through the first gas filter. Gas filtration system may comprise pressure sensor installed to determine gas pressure inside container compartment; and a computer processor, a control valve, a control element communicating with the pressure sensor and the control valve. During gas filtration gas is bubbled through liquid medium inside container compartment. In one version of implementation container may contain elastic reservoir. Bubbled gas passes from the container through the gas filter of the filtration unit. Partial vacuum is fed into gas filter so that partial vacuum facilitates gas flow through gas filter.

EFFECT: group of inventions provides higher efficiency, reliability and convenience of treatment of fluid medium containing cells or microorganisms at simultaneous reduction of material costs.

11 cl, 8 dwg