AMINOACIL-tRNA SYNTHASE FOR EFFECTIVE INTRODUCTION OF LYSINE DERIVATIVE INTO PROTEIN Russian patent published in 2023 - IPC C12N15/52 C12N15/63 C12N9/00 C12N1/21 

FIELD: biotechnology.

SUBSTANCE: mutant lysyl tRNA synthetase is provided having a mutation at position 19 of arginine (R) and/or position 29 of histidine (H) in the amino acid sequence corresponding to wild-type lysyl tRNA synthetase, wherein such mutant lysyl tRNA synthetase optionally includes a mutation at a site selected from the group consisting of isoleucine (I) at position 26, threonine (T) at position 122, leucine (L) at position 309, cysteine (C) at position 348, tyrosine (Y) at position 384 and their combinations. In addition, the following is proposed: an isolated polynucleotide encoding a mutant lysyl-tRNA synthetase; a vector for expression of the mutant lysyl-tRNA synthetase; a host cell for expressing the mutant lysyl-tRNA synthetase; expression system; a kit for obtaining a target protein containing a non-natural amino acid, as well as their use for obtaining a target protein containing a non-natural amino acid.

EFFECT: inventions make it possible to significantly increase the number of insertions of lysine derivatives and the amount of target protein containing lysine derivatives.

9 cl, 1 dwg, 1 tbl, 3 ex