FIELD: biotechnology.
SUBSTANCE: invention refers to biotechnology, namely to molecular biology, medical genetics and otorhinolaryngology. Disclosed are synthetic oligonucleotides for diagnosing (genotyping) of 35delG (rs80338939) mutation of GJB2 gene in human biomaterial. Primers are combined into kits for detecting DNA in blood and other biological materials by polymerase chain reaction.
EFFECT: invention enables highly sensitive diagnosis of congenital hereditary deafness in biological material; using this method provides also highly sensitive and objective description of human genotype with determination of homozygous and heterozygous carriage of this mutation.
1 cl, 2 dwg, 2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
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|
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|
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RU2555755C1 |
