METHOD FOR DETECTING SARS-COV-2 VIRUS BY MASS SPECTROMETRY Russian patent published in 2021 - IPC G01N33/68 

FIELD: biochemistry.

SUBSTANCE: invention relates to the field of biochemistry. A method for identifying SARS-CoV-2 virus in a sample, which includes the following steps is described: (a) sample preparation of a biological sample using the enzyme trypsin to hydrolyze proteins and obtain a mixture of polypeptides; (b) mass spectrometric analysis of the resulting mixture of polypeptides in order to identify at least two peptides selected from SEQ ID NO: 1-19; (c) if at least two peptides having the sequence SEQ ID NO: 1-19 are detected in the sample, at least one of which is a peptide selected from: SEQ ID NO: 1, SEQ ID NO: 3 - SEQ ID NO: 9, SEQ ID NO: 12 - SEQ ID NO: 19, SARS-CoV-2 virus in the sample is considered to be identified. The invention solves the problem of accelerating and increasing the reliability of the procedure for identifying the SARS-CoV-2 virus in patients. The problem is solved by the proposed method, which consists in identifying the SARS-CoV-2 virus using mass spectrometric analysis based on the developed panel of N-protein peptides (nucleocapsid).

EFFECT: accelerating and increasing reliability of the procedure for identifying the SARS-CoV-2 virus in patients.

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