ANTIBODY PURIFICATION Russian patent published in 2024 - IPC C07K1/16 C07K16/28 C07K16/06 

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to a method of purifying antibodies from impurities, such as antibody degradation products. A method of purifying an anti-BCMA/anti-CD3 bispecific antibody is proposed. The antibody preparation is injected into the loading buffer onto the hydroxyapatite (HA) resin. The antibody preparation contains an intact bispecific antibody of interest with at least one type of impurity. Wash the resin with a wash buffer containing phosphate ions at a concentration of 10 to 50 mM. Elute the intact bispecific antibody of interest from the HA resin using an elution buffer containing phosphate ions at a concentration of 40 to 100 mM. The concentration of phosphate ions is increased compared to the wash buffer so that the bispecific antibody of interest is separated from at least one type of contaminant. The pH of the loading buffer, wash buffer and/or elution buffer is between pH 7.0 and 8.0. The resulting anti-BCMA/anti-CD3 bispecific antibody preparation contains not more than 1% of trimmed bispecific antibody as an impurity.

EFFECT: obtaining a method of antibody purification.

13 cl, 7 dwg, 5 tbl, 4 ex